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Fine-mapping of an Arabidopsis cell death mutation locus 被引量：1

Fine-mapping of an Arabidopsis cell death mutation locus
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摘要 An Arabidopsis cell death mutation locus was mapped to chromosome 2 between lGS1 and mi421. The YAC clone ends, CIC9A3R, CIC11C7L, CIC2G5R and RFLP marker CDs3 within this interval, were used to probe TAMU BAC library and 31 BAC clones were obtained. A BAC contig encompassing the mutation locus, which consists of T6P5, T7M23, T12A21, T8L6 and T18A18, was identified by Southern hybridization with the BAC ends as probes. 11 CAPS and 12 STS markers were developed in this region. These results will facilitate map-based cloning of the genes and sequencing of the genomic DNA in this region. An Arabidopsis cell death mutation locus was mapped to chromosome 2 between lGS1 and mi421. The YAC clone ends, CIC9A3R, CIC11C7L, CIC2G5R and RFLP marker CDs3 within this interval, were used to probe TAMU BAC library and 31 BAC clones were obtained. A BAC contig encompassing the mutation locus, which consists of T6P5, T7M23, T12A21, T8L6 and T18A18, was identified by Southern hybridization with the BAC ends as probes. 11 CAPS and 12 STS markers were developed in this region. These results will facilitate map-based cloning of the genes and sequencing of the genomic DNA in this region.
作者 牟中林 戴亚 李家洋
出处 《Science China(Life Sciences)》 SCIE CAS 2000年第2期138-145,共8页 中国科学（生命科学英文版）
关键词 ARABIDOPSIS cell death mod1 MUTANT physical map CAPS marker. Arabidopsis, cell death, mod1 mutant, physical map, CAPS, marker.
分类号 Q754 [生物学—分子生物学]
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参考文献3

  • 1Michèle C. Heath. Apoptosis, programmed cell death and the hypersensitive response[J] 1998,European Journal of Plant Pathology(2):117～124
  • 2Sangdun Choi,Robert A. Creelman,John E. Mullet,Rod A. Wing. Construction and characterization of a bacterial artificial chromosome library ofArabidopsis thaliana[J] 1995,Plant Molecular Biology Reporter(2):124～128
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同被引文献24

  • 1X. Chen,S. Temnykh,Y. Xu,Y. G. Cho,S. R. McCouch.Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.)[J]. TAG Theoretical and Applied Genetics . 1997 (4)
  • 2H. Akagi,Y. Yokozeki,A. Inagaki,T. Fujimura.Microsatellite DNA markers for rice chromosomes[J]. Theoretical and Applied Genetics . 1996 (7)
  • 3O. Panaud,X. Chen,S. R. McCouch.Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L.)[J]. MGG Molecular & General Genetics . 1996 (5)
  • 4K. Arumuganathan,E. D. Earle.Nuclear DNA content of some important plant species[J]. Plant Molecular Biology Reporter . 1991 (3)
  • 5Chen X S,Temnykh S,Xu Y,Cho Y G,McCouch S R.Development of a microsatellite framework map providing genome-wide coverage in rice( Oryza sativa L). Theoretical and Applied Genetics . 1997
  • 6S. R. Turner,C. B. Somerville.Collapsed xylem phenotype ofArabidopsis in the secondary cell wall. The Plant Cell . 1997
  • 7R. Zhong,J. J. Taylor,Z. H. Ye.Disruption of interfascicular fiber differentiation in anArabidopsis mutant. The Plant Cell . 1997
  • 8ZhongR,YeZH.IFL,ageneregulatinginterfascicularfiberdifferentiationinArabidopsis,encodesahomeodomain_leucinezipperprotein. The Plant Cell . 1999
  • 9TaylorNG,ScheibleWR,CutlerS,SomervilleCR,TurnerSR.Theirregularxylem3locusofArabidopsisencodesacellu losesynthaserequiredforsecondarycellwallsynthesis. The Plant Cell . 1999
  • 10N. G. Tayor,S. Laurie,S. R. Turner.Multiple cellulose synthase catalytic subunits are required for cellulose synthesis inArabidopsis. The Plant Cell . 2000

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Science China(Life Sciences)
2000年 第2期

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