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Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes 被引量:1

Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes
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摘要 Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of trans-genie plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally transformed recipient. There was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. Production of co-transformed plants with greater yield and super quality was important for development of new alfalfa varieties. Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of trans-genie plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally transformed recipient. There was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. Production of co-transformed plants with greater yield and super quality was important for development of new alfalfa varieties.
机构地区 Institute of Genetics
出处 《Science China(Life Sciences)》 SCIE CAS 2000年第4期387-394,共8页 中国科学(生命科学英文版)
关键词 MEDICAGO SALIVA HNP gene ROL GENES co-transformed plant. Medicago saliva, HNP gene, rol genes, co-transformed plant.
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