摘要
AIM To clone expressed genes associated withrepair of irradiation-damaged mice intestinalgland cells treated by small intestinal RNA,andto explore the molecular mechanism ofexogenous nucleic acids improving repair ofintestinal crypt.METHODS The animal mode of test group andcontrol group was established,forty-five micebeing irradiated by γ ray were treated with smallintestinal RNA as test group,forty mice beingirradiated by γ ray were treated withphysiological saline as control group,five micewithout irradiation were used as normal control,their jejunal specimens were collectedrespectively at 6h,12h,24h,4d and 8d afterirradiation.Then by using LD-PCR based onsubtractive hybridization,these gene fragmentsdifferentially expressed between test group andcontrol group were obtained,and then werecloned into T vectors as well as beingsequenced.Obtained sequences were screenedagainst.GeneBank,if being new sequences,they were submitted to GeneBank.RESULTS Ninety clones were associated withrepair of irradiation-damaged intestinal glandcells treated by intestinal RNA.These clonesfrom test group of 6h,12h,24h,4d and 8dwere respectively 18,22,25,13,12.By screening against GeneBank,18 of which werenew sequences,the others were dramaticallysimilar to the known sequences,mainly similarto hsp,Nmi,Dutt1,alkaline phosphatase,homeobox,anti-CEA ScFv antibody,arginine/serine kinase and BMP-4,repA.Eighteen genefragments were new sequences,their acceptnumbers in GeneBank were respectivelyAF240164-AF240181.CONCLUSION Ninety clones were obtained tobe associated with repair of irradiation-damagedmice intestinal gland cells treated by smallintestinal RNA,which may be related toabnormal expression of genes and matchedproteins of hsp,Nmi,Duttl,Na,K-ATPase,alkalineph-osphatase,glkA,single strandedreplicative centromeric gene as well as 18 newsequences.
AIM To clone expressed genes associated with repair of irradiation-damaged mice intestinal gland cells treated by small intestinal RNA,and to explore the molecular mechanism of exogenous nucleic acids improving repair of intestinal crypt. METHODS The animal mode of test group and control group was established,forty-five mice being irradiated by γ ray were treated with small intestinal RNA as test group,forty mice being irradiated by γ ray were treated with physiological saline as control group,five mice without irradiation were used as normal control, their jejunal specimens were collected respectively at 6h,12h,24h,4d and 8d after irradiation.Then by using LD-PCR based on subtractive hybridization,these gene fragments differentially expressed between test group and control group were obtained,and then were cloned into T vectors as well as being sequenced.Obtained sequences were screened against.GeneBank,if being new sequences, they were submitted to GeneBank. RESULTS Ninety clones were associated with repair of irradiation-damaged intestinal gland cells treated by intestinal RNA.These clones from test group of 6h,12h,24h,4d and 8d were respectively 18,22,25,13,12.By screening against GeneBank,18 of which were new sequences,the others were dramatically similar to the known sequences,mainly similar to hsp,Nmi,Dutt1,alkaline phosphatase, homeobox,anti-CEA ScFv antibody,arginine/ serine kinase and BMP-4,repA.Eighteen gene fragments were new sequences,their accept numbers in GeneBank were respectively AF240164-AF240181. CONCLUSION Ninety clones were obtained to be associated with repair of irradiation-damaged mice intestinal gland cells treated by small intestinal RNA,which may be related to abnormal expression of genes and matched proteins of hsp,Nmi,Duttl,Na,K-ATPase, alkalineph-osphatase,glkA,single stranded replicative centromeric gene as well as 18 new sequences.
基金
"211"project fund (No.98X207)
National Natural Science Foundation of China,No.38970279
