肾癌肿瘤浸润淋巴细胞免疫组化分析及其与肾癌愈后关系研究

Analysis of TIL in RCC by immunohistochemical technique and study on relationship between TIL and prognosis of RCC

目的:了解肾细胞癌(RCC)患者抗瘤免疫状况及TIL与肾癌愈后关系。方法:应用免疫组织化学PAP法和单克隆抗体对肾细胞癌原位肿瘤浸润琳巴细胞的构成与分布及活化进行研究,检测的TIL主要表面表标志包括CD_3、CD_4、CD_8、CD_(25)和HLA-DR抗原。结果:(1)RCC患者肿瘤原位TIL以·OT细胞为主(CD_3+),其中CD_8+细胞明显高于CD_4+细胞,其CD_4+/CD_8+比值与RCC临床分期成负相关。(2)在肿瘤原位,有部分表达活化标志CD_(25)或HLA-DR抗原T细胞存在。(3)RCC患者其TIL在瘤组织中呈弥散状分布。结论 肾癌TIL反映了肾癌患者局部抗瘤免疫状况,且与分期负相关,可做为肾癌愈后的客观检测指标。

Purpose To get a comprehensive understand of antitumor immune state and relationship between TIL and prognosis of renal cell carcinoma (RCC). Methods: Employing immunohistochemical technique and monoclonal antibody, the composrtion, distribution and activation of TIL in RCC were studied in situ, the mam surface markers studied on TIL were CD3,CD4,CD8 antigens and CD25 and HLA - DR antigens , which could show the activation of T lymphocytes. Results: (1) The main part of TIL in RCC was T lymphocyte (CD:+), and among these percentage of CD8+ lymphocyles were significantly higher than that of CD4 + ones, the level of T - TIL in RCC and its CD4+/CD8+ ratio were negatively related to the level of malignity of RCC. (2) In situ, there were activated TIL to some extend. (3) TILs in RCC were distributed scatteredly in tumor tissues in situ. Conclusions: TIL in RCC was negatively related lo clinical stages of RCC. The quantitative study of TIL might be of objective standard in the prognosis of RCC- Studying TIL of RCC in situ can help us understand ami - tumor immunoreaction of RCC