摘要
0 ＩｎｔｒｏｄｕｃｔｉｏｎＢｒｅｅｄｉｎｇｐｅｓｔｒｅｓｉｓｔａｎｔｐｌａｎｔｓｕｓｉｎｇｐｌａｎｔｇｅｎｅｔｉｃｅｎｇｉｎｅｅｒｉｎｇｔｅｃｈｎｉｑｕｅｉｓａｎｅｆｆｅｃｔｉｖｅｓｔｒａｔｅｇｙｉｎｔｈｅｉｎｔｅｇｒａｔｅｄｐｅｓｔｍａｎａｇｅｍ?..
To increase the expression level of Cry IA(c) gene in transgenic plants, a plant expression vector pBinMoBc carrying the Cry IA(c) gene under control of chimeric OM promoter and Ω factor was constructed. As a control, pBinoBc carrying the CryIA(c) gene with the CaMV 35S promoter was also constructed. The vectors were transferred into tobacco plants respectively via Agrobacterium mediated transformation. ELISA assay showed that the expression level of the Cry IA(c) gene in pBinMoBc transgenic tobacco plants was 2.44 times that in pBinoBc transgenic tobacco plants, and it could be up to 0.255% of total soluble proteins. Bioassay showed that pBinMoBc transgenic tobacco plants had more notable insecticidal effect than pBinoBc transgenic tobacco plants. The above results showed that the chimeric OM promoter was a stronger promoter than CaMV 35S promoter that was widely used in plant genetic engineering, and this is very useful in pest resistant plant genetic engineering.
