乙型肝炎病毒S基因与C基因免疫小鼠诱生的免疫应答

HEPATITIS B VIRUS SURFACE AND CORE GENE-BASED VACCINE IMMUNIZATION IN BALB/C MICE

目的 观察小鼠对HBV S基因和基因疫苗的应答。方法 用已构建的HBV S基因疫苗(pCR3.1-S)和C基因疫苗(pCR3.1-C)分别给Balb/c小鼠多点肌肉注射,2wk后追加免疫一次,用ELISA法及MTT法检测小鼠血清抗体及脾细胞对HBsAg或HBcAg的特异性增殖反应。结果 免疫接种2wk后小鼠血清抗体滴度明显高于对照组,pCR3.1-C组的刺激指数明显高于pCR3.1-S注射组。结论 HBV S和C基因疫苗诱导较强的体液和细胞免疫应答强度;C基因尤以细胞免疫增高明显。

Objective To study HBV surface and core gene-based vaccine immunization in mice. Methods The HBV surface gene and core gene were cloned into an enkaryotic expression vector(pCR3. 1), respectively. The Balb/c mice were immunized intramuscularly with pCR3.1-S and pCR3.1-C. Each mouse was boosted 2 weeks after immunization. The anti-HBs and anti-HBc antibody were detected by ELISA.and the stimulatory index of splenocytes of mice immunized to HBsAg of HBcAg was measured by MTT method. Results The titers of special antibody and the stimulatory index of splenocytes from mice immunized with PCR3.1-S and pCR3.1-C were significantly higher than that of control. SI of immunized mice injected with pCR3.1-C was higher than that of pCR3.1-S group. Conclusion This study demonstrates that HBV C gene and S gene may elicit humoral and cellular immune response in mice.