清肝饮对人成纤维细胞DNA和胶原合成的影响

THE EFFECT OF QINGGANYING ON PROLIFERATION AND COLLAGEN SYNTHESES OF HUMAN LUNG FIBROBLASTS IN VITRO

目的 研究中药清肝饮对人成纤维细胞(HLF)的DNA和胶原合成的影响,从细胞水平评价清肝饮防治器官纤维化提供实验依据。方法 采用~3H-TdR和~3H-脯氨酸掺入法测定DNA和胶原合成。结果 清肝饮在1～5mg/ml浓度范围内均以浓度依赖方式抑制HLF胶原和DNA合成(P【0.05～0.01)。结论 清肝饮具有抗纤维化的作用。

Objective To investigate the potentiality of Chinese traditional medicine Qingganying on the proliferation and collagen syntheses of human lung fibroblasts(HLF). Methods The proliferation and collagen syntheses were determined by measuring the 3H-TdR and 3H-L-Proline incorporation. Results The results showed that Qingganying suppressed proliferation and collagen syntheses in a concentration - dependent manner at 1 -5mg. ml-1 (P < 0.05 - 0.01). Conclusion Qingganying could be a hopeful drugs for antifibrosis.