摘要
Objective: To investigate the role of subeschar tissue fluid (STF) in postburn cell damage. Methods: STF was collected from patients with full-thickness burn injury of 10% - 69% total body surface area (TFBSA) during operation on postburn day 2 - 4, and that from guinea pigs were obtained in escharectomy 24 h after full-thickness scald injury of 30%’TBSA was induced. Respective incubation of human umbilical vein endothelial cells (HUVEC) and hepatocytes with STF were performed for the observation of the effects of STF on the activity, morphology, physiological function and monolayer cell permeability of HUVEC, and the percentages of apoptotic and necrotic hepatocytes were determined. Results: (l) STF had evident deleterious effect on endothelial cells. (2)are could induce apoptosis and necrosis of hepatocytes. Conclusion: STF plays an important role in causing early postburn damages.
Objective: To investigate the role of subeschar tissue fluid (STF) in postburn cell damage. Methods: STF was collected from patients with full-thickness burn injury of 10% - 69% total body surface area (TFBSA) during operation on postburn day 2 - 4, and that from guinea pigs were obtained in escharectomy 24 h after full-thickness scald injury of 30%'TBSA was induced. Respective incubation of human umbilical vein endothelial cells (HUVEC) and hepatocytes with STF were performed for the observation of the effects of STF on the activity, morphology, physiological function and monolayer cell permeability of HUVEC, and the percentages of apoptotic and necrotic hepatocytes were determined. Results: (l) STF had evident deleterious effect on endothelial cells. (2)are could induce apoptosis and necrosis of hepatocytes. Conclusion: STF plays an important role in causing early postburn damages.
