北京地区汉族妇女的雌激素受体基因多态性分布

Polymorphism of estrogen receptor gene from Chinese Han women in Beijing

目的 研究雌激素受体(ER)基因多态性在中国北京地区汉族妇女中的分布以及与其他种族的差异。方法 通过聚合酶链式反应-限制性片段长度多态性 (PCR-RFLP) 方法研究ER基因的Xba Ⅰ和Pvu Ⅱ的酶切多态性分布。结果 中国北京地区汉族妇女的ER基因型Xba Ⅰ和Pvu Ⅱ的酶切多态性分布为ppxx:7(4.8％),PPXx:10(6.8％),PPxx:3(2.1％),PpXX:2(1.4％),PpXx:51(34.9％),Ppxx:36(24.7％),ppXX:1(0.7％),ppXx:9(6.2％),ppxx:27(18.5％)。结论 检测到中国北京地区汉族妇女ER基因Xba Ⅰ和Pvu Ⅱ相结合的所有9种多态性分布,以Pp Xx以及Ppxx型最为常见。

Objective: To investigate the polymorphism of estrogen receptor gene from Chinese Han women in Bei jing. Methods Estrogen receptor gene polymorphism of 146 Chinese Han women in Beijing were analyzed with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The genotype frequencies at Xha I and Pvu II sites of ER genes of Chinese Han women in Beijing were as following: PPXX: 7(4.8% ) ,PPXx: 10(6.8% ),PPxx: 3(2.1 %),PpXX:2(1.4% ) ,PpXx:51(34.9% ),Ppxx: 36(24.1% ),ppXX: 1(0.7% ),ppXx: 9(6.2% ),ppxx: 27(18.5%). Conclusions All nine genotypes of ER gene at Xba 1 and Pvu Ij sites were found in Chinese Han women of Beijing,and genotypes PpXx and Ppxx were dominant among them.