解脲支原体宫内感染与胎膜早破关系的研究

Cinical study on relationship between intrauterine infection by ureaplasma ureatytiam and premature rupture of membrane

目的 探讨解脲支原体(UU)宫内感染与胎膜早破的相关性。方法 运用培养法对妊娠晚期32例胎膜早破孕妇(试验组)和20例正常孕妇(对照组)的剖宫产术中羊水进行支原体培养检测,评价6种抗菌药物对抑制支原体的敏感性;同时运用PCR-微板核酸分子杂交法对上述羊水进行解脲支原体DNA检测。结果 试验组分离培养检测的UU感染阳性率为28.1％,对照组UU感染阳性率为5.0％(P>0.05);6种抗生素药敏试验提示氧氟沙星药物敏感性最弱;在同时进行的PCR-微板核酸分子杂交法试验中,试验组的UU检出率为46.9％,对照组的UU检出率为10.0％,两组比较差异有显著性(P<0.01)。结论 解脲支原体所致的宫内感染是部分妊娠晚期胎膜早破的原因之一。PCR-微板核酸分子杂交法联合培养法,是诊断解脲支原体宫内感染的特异、快速和准确的方法。

Objective To study the relationship between intrauterine infection by ureaplasma urealyticum (UU) and premature rupture of the membrane (PROM) .Methods Myocplasma cultures of amniotic fluid were performed in 32 cases of cesarean section due to PROM (study group),and 20 cases of normal pregnancies with cesarean section (control group) . The susceptibility of six antibiotic agents to UU was determined. UU DNA detections of all cases were done by PCR micro-panel DNA hybridization.Results The UU positive rate of study group,which was detected by culture of amniotic fluid in PROM cases,was 28.1% versus 5.0% of control group ( P > 0.05) . With PCR micro-panel DNA hybridization method,there was a significant difference between the two groups (P < 0.01),and the positive rate of study group was 46.9% versus 10.0% of control group. The susceptibility of Ofioxacin to UU was relatively low compared with that of the other five antibiotic agents. Conclusions Some cases of premature rupture in the late period of pregnancy were the result of UU intrauterine infection. Culture of the amniotic fluid together with PCR micro-panel DNA hybridization is a fast,specific and accurate method to diagnose UU intrauterine infection.