孕激素受体A、B亚型在子宫肌瘤中的表达

Expression of human progesterone receptor isoforms A and B in uterine leiomyoma

目的 旨在研究两种孕激素受体亚型 (hPR-A和hPR-B) 在子宫肌瘤和正常子宫肌层的分布及其mRNA的比例,探讨其在子宫肌瘤发生、发展中的意义。方法 选取22例因子宫肌瘤行全子宫切除术的标本,每1例取肌瘤组织和正常肌层组织,分别制成石蜡切片和冰冻标本。前者经免疫组化定位研究孕激素受体亚型;后者用于提取RNA,通过逆转录-多聚酶链反应,半定量研究孕激素受体亚型的mRNA表达。结果 免疫组化显示子宫肌瘤和正常肌层细胞的细胞核中,可见hPR及hPR-B的阳性颗粒;hPR-A、hPR-B和hPR-(A+B)的mRNA在肌瘤的表达量均高于周围正常肌层。无论在子宫肌瘤还是在正常肌层组织中,hPR-A与hPR-B的mRNA表达量无明显差异。结论 hPR-A、hPR-B为核受体;A亚型和B亚型的mRNA在子宫肌瘤表达高于正常肌层;孕激素受体亚型可能与子宫肌瘤的发生有关。

Objective Human progesterone receptor (hPR) exists two isoforms,hPR-A and hPR-B,which are structurally and functionally different. To investigate the role of the two isoforms in the development and progression of uterine leiomyoma. Tlie location and the messenger ribonucleic acid (mRNA) expressions of the two isoforms in leiomyoma and normal myometrium were detected respectively. Methods The tissues of leiomyoma and normal myometrium from 22 uteri,which were excised for leiomyoma ,were used for the localization of the above isoforms by immunohistochemistry. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the relative concentrations of the hPR isoforms. Results Both hPR-A and hPR-B were nuclear receptors. There are more hPR-A. hPR-B and hPR-(A + B) in mRNA of leiomyoma than those of normal myometrium. hPR-A and hPR-B in mRNA expressed equally in leiomyoma and normal myometrium. Conclusions Both hPR-A and hPR-B were nuclear receptors. hPR-A and hPR-B of mRNA were more in leiomyoma than those in normal myometrium. The development of uterine leiomyoma seems related with the progesterone receptor isoforms.