老年人自然免疫状态下抗肺炎链球菌抗体的检测

Streptococcus pneumoniae capsular polysaccharide antibodies of old people in naturally acquired active immunity

目的探讨自然免疫状态下血清抗肺炎链球菌荚膜多糖特异性抗体值,为老年人是否需常规使用PPV23免疫提供参考。方法健康人85例(健康组),分为青年、中年、老年组;老年COPD患者18例(COPD组),ELISA检测血清特异性抗体。结果健康组:IgA老年组同中、青年组差异无统计学意义;IgM青年组高于其他两组(P<0.05);总IgG差异无统计学意义,IgG4老年及中年组高于青年组(P<0.05)。IgM浓度与年龄负相关(r=-0.274,P<0.05),IgG4与年龄正相关(r=0.342,P<0.05)。COPD组和健康老年人组差异无统计学意义。结论未发现健康及COPD老年人血清抗肺炎链球菌保护性抗体整体水平低下,不支持该人群常规使用PPV23。

Objective To explore the necessary for elderly individuals to immunize with pneumococcal vaccine polyvalent,antibodies of several isotypes and subclasses that specific to streptococcus pneumoniae capsular polysaccharide（CPS） in serum.Methods The specific antibodies in serum were measured in 85 health adults and 18 elderly patients with chronic obstructive pulmonary disease（COPD） by indirect Enzyme Linked Immunosorbent Assay（ELISA）.Results The health adults were split into aged,meddle-aged and young groups.The level of IgA in aged group was not lower than other two groups in statistics（P0.05）,the levels of IgM in aged group and middle-aged group were both significantly lower than that those in young group while it was opposite in the level of IgG4（P0.05）.The differences of total IgG between elderly group and other two groups were no statistically significant（P0.05）.The level of IgM in health individuals negatively correlated with age（r=-0.274,P0.05） while the level of IgG4 positively correlated with age（r=0.342,P0.05）.The differences of all measured antibodies between elderly COPD patients and health aged person were no statistically significant（P0.05）.Conclusions There is no significant decline on overall levels of the protective antibodies in serum of health aged individuals and elderly patient with COPD,so it does not support introducing pneumococcal vaccine polyvalent to those populations as common practice.