龈沟液中可溶性细胞间粘附分子-1的检测

Measurement of Soluble Intercellular Adhesion Molecule 1 in Gingival Crevicular Fluid

目的：探讨龈沟液中可溶性细胞间粘附分子１（ｓＩＣＡＭ１）的浓度与牙周病的相互关系。方法：采用常规滤纸条法收集牙周健康者（Ｈ） 、边缘性龈炎（ＭＧ） 以及慢性成年人牙周炎（ＣＡＰ）患者龈沟液样本，用ＥＬＩＳＡ法检测各样本中ｓＩＣＡＭ１ 的含量。结果：３ 组受检牙龈沟液中ｓＩＣＡＭ１ 的含量不同，其中ＭＧ组和ＣＡＰ组的ｓＩＣＡＭ１ 水平明显高于Ｈ组（ Ｐ＜ ０．０１） ；龈沟液中ｓＩＣＡＭ１ 的水平与牙龈出血指数（ＢＩ）、牙周探诊深度（ＰＤ）、菌斑指数（ＰＬＩ） 呈等级相关关系（ Ｐ＜ ０．００１）。结论：龈沟液中ｓＩＣＡＭ１

Objective:To detect the levels of soluble intercellular adhesion molecule 1(sICAM 1) in gingival crevicular fluid(GCF) and to evaluate relationship between GCF sICAM 1 levels and periodontal diseases. Methods: Following careful sampling of GCF with standardised filter strips in healthy patients(H) and patients with marginal gingivitis(MG), or chronic adult periodontitis(CAP), the levels of sICAM 1 shed into the GCF were detected by ELISA technique. Results: The sICAM 1 levels of GCF among three groups were different. GCF sICAM 1 levels were obviously higher in MG and CAP group than in H group (P<0 01). There was a positive correlation between sICAM 1 levels and bleeding index(BI), probing depth(PD), plaque index(PLI) (P<0 001). Conclusion:These results suggest that GCF sICAM 1 levels, as a sensitive and objective marker, may be useful to assess the inflammatory phenomena in periodontal tissues.