人体增生性疤痕移植于裸鼠动物模型的建立及稳定性观察

Establishment of Animal Model and Observation of Stability on the Implant of Hypertrophic Scar into the Nude Mouse

目的：将增生性疤痕组织块以不同的方法移植于裸鼠，建立一稳定的疤痕动物模型，为研究增生性疤痕提供实验基础。方法：将３ ｍｍ ×３ ｍｍ 增生性疤痕组织块分别移植于裸鼠皮下和体表创面，定期进行大体观察，组织学观察及胶原定量测定。结果：增生性疤痕组织块移植于裸鼠皮下后，移植物可成活８０～９０ ｄ，无排斥反应，并保留原增生性疤痕特性；移植物体积随着移植时间而逐渐增加。结论：增生性疤痕组织块移植于裸鼠皮下建立的增生性疤痕动物模型是一成功、稳定的动物模型，为疤痕的研究提供了可靠的基础。应用该动物模型时，作者建议把移植后３～１０ 周的动物作为增生性疤痕的标准动物模型。

Objective: To implant some pieces of human hypertrophic scar into subcutaneous pockets of nude (athymic) mice by different methods and to establish a steady scar animal model for studying hypertrophic scar of human beings. Methods: Pieces of hypertrophic scar 3 mm×3 mm were transplanted into subcutaneous pockets and excised regions(the surface of the wounds). The changes of general condition, the histotypic and collagen content of hypertrophic scar were observed termly. Results: The implants were carried 80～90 d. No rejection phenomena were observed. All implants retained their original histotypic character. Volume measurements suggests there was a size increase of the implants with time. Conclusions: Use of the nude mouse for implants of hypertrophic can establish a steady hypertrophic scar experimental model for further studies in human beings. It is also suggested that the standard scar animal model may be at 3～10 weeks after implanted.