摘要
Objective To investigate the relationship between the pathogenesis of Guillain Barre syndrome (GBS) and Campylobacter jejuni (C jejuni) in China Methods C jejuni strains were isolated from fresh stools of 47 GBS patients in Beijing area from 1995 to 1997 by modified Skirrow’s method Serotyping of C jejuni was performed with Penner’s method in 47 GBS patients and 171 patients with C jejuni enteritis in our hospital during the same period The stools from which no C jejuni strains could be isolated were typed by PCR RFLP (restriction fragment length polymorphism) method Results Six C jejuni strains were isolated by Skirrow’s method, including 3 strains of Penner serotype 2, 1 Penner serotype 4, 1 Penner serotype 19 and 1 Penner serotype 26 Three strains of DNA Ⅰ, which was equal to the same type of Penner serotype 19, were found by PCR RFLP method Conclusion C jejuni isolated from Beijing area are similar to those reported in literature, which once more confirms the molecular mimicry pathogenetic theory of GBS caused by C jejuni infection in China The similarities of serotypes between C jejuni strains from GBS patients and those from C jejuni enteritis patients may explain the high incidence of GBS following C jejuni infection in China
Objective To investigate the relationship between the pathogenesis of Guillain Barre syndrome (GBS) and Campylobacter jejuni (C jejuni) in China Methods C jejuni strains were isolated from fresh stools of 47 GBS patients in Beijing area from 1995 to 1997 by modified Skirrow’s method Serotyping of C jejuni was performed with Penner’s method in 47 GBS patients and 171 patients with C jejuni enteritis in our hospital during the same period The stools from which no C jejuni strains could be isolated were typed by PCR RFLP (restriction fragment length polymorphism) method Results Six C jejuni strains were isolated by Skirrow’s method, including 3 strains of Penner serotype 2, 1 Penner serotype 4, 1 Penner serotype 19 and 1 Penner serotype 26 Three strains of DNA Ⅰ, which was equal to the same type of Penner serotype 19, were found by PCR RFLP method Conclusion C jejuni isolated from Beijing area are similar to those reported in literature, which once more confirms the molecular mimicry pathogenetic theory of GBS caused by C jejuni infection in China The similarities of serotypes between C jejuni strains from GBS patients and those from C jejuni enteritis patients may explain the high incidence of GBS following C jejuni infection in China
