摘要
Objective To investigate the dose effect of dietary L arginine supplementation on burn wound healing in rats Methods 218 Sprague Dawley rats (weighing 200-250?g) were subjected to 10% deep partial thickness scald burns and were randomized into six groups Groups A, B, C, D, E and F received 800, 400, 200, 100, 50 and 0?mg·kg -1 ·d -1 L arginine in the form of L arginine solution, and 0, 727, 1090, 1272, 1364, and 1454?mg·kg -1 ·d 1 glycine, respectively Each solution was isonitrogenous The times of completing re epithelization were recorded The contents of hydroxyproline (OHP) in burn wound area (index of reparative collagen synthesis) and the ratios of type Ⅰ and type Ⅲ collagen were examined in all groups Results The times of completing re epithelization (day) in groups A, B, C, D, E, and F were 24 9±1 95, 22 5±2 0, 20 2±2 4, 23 5±2 6, 23 8±3 5, and 24 7±2 3, respectively The contents of hydroxyproline in groups B, C and D were higher than in groups A, E and F on PBD 7, 10 and 14 The ratios of typeⅠ and type Ⅲ collagen in groups B, C and D were lower than in groups A, E and F Conclusion Oral dietary L arginine supplementation from 100?mg·kg -1 ·d -1 to 400?mg·kg -1 ·d -1 shortened the times of re epithelization, increased amounts of hydroxyproline, and accelerated the synthesis of reparative collagen in burn rats
Objective To investigate the dose effect of dietary L arginine supplementation on burn wound healing in rats Methods 218 Sprague Dawley rats (weighing 200-250?g) were subjected to 10% deep partial thickness scald burns and were randomized into six groups Groups A, B, C, D, E and F received 800, 400, 200, 100, 50 and 0?mg·kg -1 ·d -1 L arginine in the form of L arginine solution, and 0, 727, 1090, 1272, 1364, and 1454?mg·kg -1 ·d 1 glycine, respectively Each solution was isonitrogenous The times of completing re epithelization were recorded The contents of hydroxyproline (OHP) in burn wound area (index of reparative collagen synthesis) and the ratios of type Ⅰ and type Ⅲ collagen were examined in all groups Results The times of completing re epithelization (day) in groups A, B, C, D, E, and F were 24 9±1 95, 22 5±2 0, 20 2±2 4, 23 5±2 6, 23 8±3 5, and 24 7±2 3, respectively The contents of hydroxyproline in groups B, C and D were higher than in groups A, E and F on PBD 7, 10 and 14 The ratios of typeⅠ and type Ⅲ collagen in groups B, C and D were lower than in groups A, E and F Conclusion Oral dietary L arginine supplementation from 100?mg·kg -1 ·d -1 to 400?mg·kg -1 ·d -1 shortened the times of re epithelization, increased amounts of hydroxyproline, and accelerated the synthesis of reparative collagen in burn rats
