激光共聚焦显微镜观察磷酸肌酸促缺氧心肌细胞钙离子通道恢复的作用

Obeservation of the Improvement of Ca 2+ Channal's Recovery Incubation with Creatine Phosphate by Laser Scanning Microscope

目的用激光共聚焦显微术探讨缺氧过程中心肌细胞损伤的离子机制。方法取大鼠心脏分离心肌细胞，实验组和对照组各２０份，分别在含磷酸肌酸和无磷酸肌酸的培养基中孵育２４～７２ｈ，ｆｌｕｏ３ＡＭ染色，４８８ｎｍ激光激发，选择固定视野以３３ｍｓ间隔记录３ｍｉｎ，分析荧光强度变化规律。结果标本荧光呈周期性变化，对照组周期长，且不稳定，实验组明显缩短，周期稳定。结论磷酸肌酸具有对缺氧心肌的保护作用。

Purpose To ascertain the ionic mechanism of myocardial injury after by means of confocal laser scanning microscope. Methods Myocardial cells,which were derived from rat myocardium dissociated with collagenase and hyaluronidase,were cultivated in the medium either with or without creatine phosphate(CP) for 24 to 72 hours,during which a hypoxic environment was made in the first 5 hours.After 24 or 72 hours incubation myocardial cells were loaded with flou 3AM,a Ca 2+ marker,and observed under a confocal laser scanning microscope . Results The fluorescence intensity was showing a rhythmical change.The frequency of the rhythm was stable and high in the specimen after 72 hours cultivation and in the CP group it became stable earlier and higher than that of the control group. Conclusions Creatine phosphate proves myocardial protective and antiarrythmic.