三个抗锈基因Lr37、Sr38和Yr17分子标记的筛选与改良

Development of molecular markers for three rust resistance genes Lr37, Sr38 and Yr17

含有偏凸山羊草2NS染色体的普通小麦易位系带有三个紧密连锁的抗锈基因Lr37、Sr38和Yr17。以内切酶EcoRI和分子探针Xm wg682为基础,发现来自偏凸山羊草2NS的易拉片断在1.8kb 处有一条独特的RFLP标记。通过DNA克隆和测序,这条RFLP被成功地转换成为引物UI和LN1为基础的PCR标记。回交后代的鉴定结果表明,

There are 3 closely linked rust resistance genes,i e Lr37,Sr38 and Yr17,in wheat translocation lines containing the 2NS segment from Ae. ventricosum. Restriction enzyme EcoRI combined with molecular probe Xmwg682 showed a strong 1 8kb RFLP marker for the Ae. ventricosum segment of chromosome 2NS. Through DNA cloning and sequencing, this RFLP marker was successfully transfered into a PCR marker, which was based on the primers U1 and LN1. Experimental results based on backcross population indicated that the sensitivity and specificity of PCR marker were almost as same as that of RFLP marker.