75g/LNaCl/65gL右旋糖酐抗高原创伤失血性休克大鼠的作用

Effect of 75g/L Hypertonic Saline/60 g/L Dextran-40 in Traumatic-Hemorrhagic Shock Rats at High Altitude

目的 探讨质量浓度为 75g LNaCl 质量浓度为 6g L右旋糖酐 (HSD)对高原 (西藏拉萨高原现场 )创伤失血性休克的急救作用及其量效关系。方法 初进高原大鼠 4 4只 ,分为等渗盐水对照组 (11只 ) ,HSD 4ml kg、6ml kg、8ml kg治疗组 (各 11只 ) ,动物用戊巴比妥钠腹腔麻醉 ,右侧股骨粉碎性骨折加放血 (45mmHg维持 70分钟 )复制创伤失血性休克模型 ,观察静脉一次输注上述几个剂量HSD对创伤失血性休克大鼠血流动力学指标和动物存活时间的影响 ,以等渗盐水作对照。结果 一次性静脉输注HSD 4 ,6,8ml kg均能显著升高休克大鼠左室内压 (LVSP)、改善包括平均动脉压 (MAP)、左室内压最大变化速率 (±dp dtmax)、心肌最大收缩速度 (Vpm)、心肌收缩向量环面积 (Lo)在内的血流动力学指标 ,维持时间超过 2小时 ,延长休克动物的存活时间 ,治疗组 6ml kg和 8ml kg较 4ml kg效果更好。结论 HSD 4～ 8ml kg对高原创伤失血性休克大鼠有较好的早期急救作用 ,剂量以 6～ 8ml kg较为合适。

s Objective 75g/L NaCl/60 g/Ldextran-40(HSD) was effective in traumatic shock resuscitation at plain or simulated high altitude in hypobaric hypoxic chamber.This study was designed to evaluate the resuscitation effect and dose-effect relationship of 75g/L NaCl/60 g/Ldextran-40(HSD g/ml) on traumatic-hemorrhagic shock rats at high altitude (Lahsa , Tibet ). Methods Forty-four rats were randomly divided into four groups(n=11 in each group):group I: control group; group II: 4ml/kg HSD; group III: 6ml/kg HSD;group IV: 8ml/kg HSD.Traumatic-hemorrhagic shock model was made by right femur fracturing and bleeding to an arterial pressure of 6.0kPa for 70 minutes. Rats received a bolus injection of different dosage of HSD mentioned above respectively; 4ml/kg of 9g/L NaCl was used as the control.The influence of HSD on hemodynamic parameters and survival time were observed.Results HSD 4ml/kg、 6ml/kg or 8ml/kg of HSD could significantly increase left ventricular systolic pressure (LVSP), improve hemodynamic parameters including mean arterial pressure(MAP) , maximal rate of left ventricular pressure rise and drop( dp/dtmax), maximal physiological velocity of myocardium (Vpm), area of P-dp/dtmax vector loop (Lo), and significantly prolong the survival time of shock rats.Among the three treated groups, HSD of 6ml/kg or 8ml/kg had satisfactory effects. Conclusions HSD has effects in treatment of traumatic-hemorrhagic shock rats at high altitude . The appropriate dosage is 6-8ml/kg. Key word High altitude Shock,traumatic,hemorrhagic Dextranse of Guanhua Hospital, Shanghai 200054) ABSTRACT Aim: To investigate the effects of coxsackievirus (CVB 3) on ion channel currents in rat cardiomyocytes. Methods: Rat cardiomyocytes were isolated with collagenase to acquire single cardiomyocyte, the currents of VDCC, Na + and K + were recorded by patch clamp whole cell techniques.Results:CVB 3 infection increased the VDCC current and outward potassium current(I out ), while decreased inwardly rectifying potassium current(I KI ); but it didn't affect the Na + current significantly. Conclusion: The effects of CVB 3 on L type VDCC current、I out 、 I KI may be the cause of cell damage and the occurrence of abnormal electric activities induced by CVB 3 infection. To investigate the effects of coxsackievirus (CVB 3) on ion channel currents in rat cardiomyocytes. Methods: Rat cardiomyocytes were isolated with collagenase to acquire single cardiomyocyte, the currents of VDCC, Na + and K + were recorded by patch clamp whole cell techniques.Results:CVB 3 infection increased the VDCC current and outward potassium current(I out ), while decreased inwardly rectifying potassium current(I KI ); but it didn't affect the Na + current significantly. Conclusion: The effects of CVB 3 on L type VDCC current、I out 、 I KI may be the cause of cell damage and the occurrence of abnormal electric activities induced by CVB 3 infection. Methods: Rat cardiomyocytes were isolated with collagenase to acquire single cardiomyocyte, the currents of VDCC, Na + and K + were recorded by patch clamp whole cell techniques.Results:CVB 3 infection increased the VDCC current and outward potassium current(I out ), while decreased inwardly rectifying potassium current(I KI ); but it didn't affect the Na + current significantly. Conclusion: The effects of CVB 3 on L type VDCC current、I out 、 I KI may be the cause of cell damage and the occurrence of abnormal electric activities induced by CVB 3 infection. Rat cardiomyocytes were isolated with collagenase to acquire single cardiomyocyte, the currents of VDCC, Na + and K + were recorded by patch clamp whole cell techniques.Results:CVB 3 infection increased the VDCC current and outward potassium current(I out ), while decreased inwardly rectifying potassium current(I KI ); but it didn't affect the Na