HPLC法测定复方乳酸诺氟沙星制剂中乳酸诺氟沙星和乳酸甲氧苄氨嘧啶的含量

Simultaneous determination of norfloxacin lactas and trimethoprimum lactas by HPLC

采用高效液相色谱法，以水乙腈三乙胺（３２５∶１７５∶２２ ，用磷酸调ｐ Ｈ 至３ ．０） 为流动相，在λ＝ ２４０ ｎｍ 进行检测。该方法可使乳酸诺氟沙星和乳酸甲氧苄氨嘧啶达到良好分离。该方法操作简便、灵敏、重现性好。

A rapid specific and sensitive HPLC method for simultaneous determination of norfloxacin lactas and trimethoprimum lactas was developed.Using a Zorbax ODS column(250 mm×4.6 mm)under room temperature,the mobile phase consisted of water acetonitrile triethylamine(325∶175∶2 2,adjusted to pH 3.0 with phosphoric acid)and flow rate was 1.0 ml/min.The UV detection wavelength was 240 nm.The linear range of determination is 100～300 mg/L for norfloxacin lactas and 25～75 mg/L for trimethoprimum lactas.This method is simple,sensitive and stable.