化学发光法测定胃粘膜氧自由基的探讨

Study of Detecting OFR in Antral Mucosa by Chemiluminescent Assay

探讨用化学发光法测定胃粘膜氧自由基（ＯＦＲ） 及用抑制试验进行分类． 对３０ 例Ｈｐ （ ＋ ）溃疡病患者在抗Ｈｐ 治疗前后３ 个月均做胃粘膜ＯＦＲ 测定． 根治Ｈｐ 后ＯＦＲ 明显下降． 对发光数２０００ 以上的标本用抑制试验进行ＯＦＲ 分类检测， 结果表明： 化学发光法能简便、灵敏地检测胃粘膜中ＯＦＲ含量．

To study the chemiluminescent assay and inhibitory test in detection of oxygen free radical (OFR) in antral mucosa.Methods:30 Hp(+) patients with peptic ulcer were enrolled.Endoscopic examination and OFR detection in mucosa were carried out before and three months after anti-Hp therapy.Results:The value of OFR was significantly decreased after eradication of Hp (from 114 38 counts/min/mg to 36 53 counts min/mg,P<0 01).Inhibitory test showed that there was 72 9% of hypochlorous acid,37% of hydroxyl radical,32% of superoxide and 31% of hydrogen perooxide.Conclusion:Chemiluminescent assay is a simple and sensitive method for detection of OFR in mucosa and available for study the mechanism of diseases caused by OFR.