Ca^(2+)和Tb^(3+)对白眉蝮蛇蛇毒磷脂酶A_2荧光光谱的影响

Effects of Ca^(2+) and Tb^(3+) on the Fluorescence Spectrometry Phospholipase A_2 Isolated from Chinese Agkistrodon Blomhoffii Ussurensis Venom

研究了金属离子Ｃａ２ ＋ 和Ｔｂ３ ＋ 对长白山白眉蝮蛇蛇毒磷脂酶Ａ２（ｐｈｏｓｐｈｏｌｉｐａｓｅ Ａ２） 荧光光谱的作用， 发现Ｃａ２ ＋ 浓度的增加能够增强磷脂酶Ａ２ 的荧光发射强度． 而且Ｃａ２ ＋ 浓度的增大能够明显加快磷脂酶Ａ２ 与其相应反应底物ＤＰＰＣ的反应速率． 稀土离子Ｔｂ３ ＋ 在低浓度条件下对磷脂酶Ａ２ 起荧光淬灭作用， 而在浓度较高时能够提高磷脂酶Ａ２ 荧光发射强度．

The project of this study was to research the effects of the Ca 2+ and Tb 3+ on the fluorescence spectra of phospholipase A 2 isolated from Chinese \%Agkistrodon blomhoffii Ussurensis\% venom.The results showed that the increase of Ca 2+ ion can improve the emission fluorescence intensity of phospholipase A 2 .And the reaction between the enzyme and its substrate(DPPC) could be accelerated by Ca 2+ .At lower concentrations,the Tb 3+ ion could quench the fluorescence of phospholipase A 2 ;but at higher concentrations it could increase the fluorescence of this enzyme.