同步荧光法研究白眉蝮蛇蛇毒酶发色团的微环境

Synchronous Fluorescence Spectra Study on Four Kinds of Enzymes Isolated from Chinese Agkistrodon Blomhoffii Ussurensis Venom

利用同步荧光技术对长白山白眉蝮蛇蛇毒中纯化得到的４ 种酶： 磷脂酶Ａ２ （ｐｈｏｓｐｈｏｌｉｐａｓｅＡ２ ， ＰＬＡ２） 、精氨酸酯酶（ａｒｇｉｎｉｎｅ ｅｓｔｅｒａｓｅ，ＡＥａｓｅ） 、纤溶酶（ｆｉｂｒｉｎｏｌｙｔｉｃ ｅｎｚｙｍｅ，ＦＥａｓｅ） 和Ｌ－ 氨基酸氧化酶（Ｌ－ａｍｉｎｏ ａｃｉｄ ｏｘｉｄａｓｅ， Ｌ－ ａ ａ ｏｘｉｄａｓｅ） 进行了研究． 结果表明： 当Δλ＝ ２０ｎｍ 时， 酶的荧光主要由酪氨酸（Ｔｙｒ） 残基所贡献； 当Δλ≥７５ｎｍ 时， 酶的荧光主要由色氨酸（Ｔｒｐ） 所贡献． 而且， 长白山白眉蝮蛇蛇毒４ 种酶的Ｔｙｒ 和Ｔｒｐ 残基所处的微环境并不相同．

Four kinds of enzymes isolated from Chinese \%Agkistrodon Blomhoffii Ussurensis \%venom were studied by synchronous fluorescence spectrometry.The results showed that at Δλ=20 nm the enzyme emission florescence spectra were mainly contributed by Tyr residues.And the emission fluorescence of the enzymes were mainly contributed by Trp residues at Δλ≥75 nm.The microenviroments in which the fluorchrones (mainly Tyr and Trp residues) exist in the four kinds of enzymes are apparent different.