荧光染料离子缔合作用在荧光法测定微量DNA中的应用

Association of Dyes Bearing with Opposite Charges and Its Application in the Determination for Micro Amounts of Nucleic Acids

建立了高灵敏荧光分光光度测定ＤＮＡ 和ＲＮＡ 的新方法． 方法的线性范围分别为０－０４ ～１－２０μｇ／ｍ Ｌ（ 鲑鱼精子ＳＭＤＮＡ 和小牛胸腺ＣＴＤＮＡ） ， ０－１０ ～１－２０μｇ／ｍＬ（ 酵母ＲＮＡ） ， 检测限分别为１７ｎｇ／ｍＬ（ＳＭＤＮＡ） ， ２４ｎｇ／ｍＬ（ＣＴＤＮＡ） ， ９８ｎｇ／ｍＬ（ＲＮＡ） ． 将方法用于实际样品金黄色葡萄球菌中ＤＮＡ 含量的测定， 结果满意．

It has been found that the fluorescence of tetrasulfonated aluminum phthalocyanine was obviously quenched by cationic dyes,but recovered in the presence of nucleic acid. Based on this phenomenon,a novel method for the determination of nucleic acids was investigated.The method presented is senstive and with simplicity in manipulation.Besides,since a fluorescent dye emitting at long wavelength region is employed,this method has the advantage of effectively reducing the interference from background fluorescence and scattering light.