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仿生荧光免疫分析卵清白蛋白 被引量:4

Biomimetic Fluoroimmunoassay for Egg Albumin
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摘要 利用分子印记技术, 制备了卵清白蛋白(EA) 的人工抗体. 研究了其对抗原的特异性吸附能力, 在此基础上, 模拟竞争型免疫反应, 建立了卵清白蛋白的仿生荧光免疫分析方法. A gel mimetic antibody for egg albumin has been prepared by molecular imprinting and applied to develop a biomimetic fluoroimmunoassay for egg albumin. The format of the assay is analogous to that of the competitive fluoroimmunoassay. The specific binding between the mimetic antibody and antigen(egg albumin) was tested by using egg albumin-FITC,bovine serium albumin(BSA)-FITC and human serium albumin(HSA)-FITC conjugates as fluorescence probes,the results are satisfactory.
机构地区 厦门大学化学系
出处 《福州大学学报(自然科学版)》 CAS CSCD 1999年第S1期116-117,115,共3页 Journal of Fuzhou University(Natural Science Edition)
关键词 分子印记 模拟抗体 仿生荧光免疫分析 卵清白蛋白 fluoroimmunoassay molecular imprinting egg albumin
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