光谱检测新技术应用研究—Ⅰ.吸光标记染料的测定

Study on the Application of Novel Spectrometric Technique--Ⅰ.Determination of Labeling Dyes

将所提出的光谱检测新技术应用于染料标记蛋白质的分析测定， 所得光谱图形与吸收光谱相似， 检测限比相应的分光光度法测定降低１ 个数量级， 并利用等摩尔连续变化法和摩尔比法测定了染料与蛋白质的配位比．

The novel spectrometric technique proposed applied to study labeling dyes.The spectral profiles gained by this technique were all similar to the corresponding UV-Vis absorption spectra,and the detection limits of dyes by this method were10 times lower than those by spectrophotometry.