一种DNA的微量荧光分析新方法

A New Fluorescent Analysis Method for Trace DNA

建立了用１０ ，１０′－ 二乙基－ ２ ，２′－ 二磺酸基－ ９ ，９′双吖啶（ＤＥＤＳＢＡ） 检测微量ＤＮＡ 荧光分析新方法， 其线性范围为１－０ ～１０－０ ｍｇ／Ｌ； 检测限为０－４６ ｍｇ／Ｌ； 测定浓度为５－０ ｍｇ／Ｌ； ＤＮＡ 溶液的相对标准偏差为０－８ ％ ．

A new fluorescent analysis method for trace DNA by DEDSBA was developed.The linear range for the determination of DNA was 1 0～10 0 μg/mL.The determination limit is 0 46μg/mL. The relative standard deviation (N=10) at an analytical concentration of 5 0μg/mL of DNA is 0 8%.