荧光动力学法测定痕量草酸根

Determination of Trace Oxalate by Kinetic-fluorimetric Method

基于在高氯酸溶液中痕量草酸根对铁（ Ⅲ） 催化过氧化氢氧化罗丹明６Ｇ 反应的抑制作用，建立了动力学荧光法测定草酸根的新方法． 方法的检出限为９３μｇ／Ｌ， 线性范围为０－１ ～１－２ｍｇ／Ｌ．将方法用于人体尿液、菠菜中草酸含量的测定， 结果满意．

Anew kinetic- fluorimetricinhibiting method for the determination oftrace oxalate is proposed . The methodis based on theinhibition effectofoxalate on thecatalytic oxidation reaction ofrhodamine6G- hydrogen peroxidebyiron (Ⅲ)in perchloricacidsolution . Thedetectionlimitfor oxalateis9-3 ×10- 2μg/mL . Thelinearrange ofthe determination is 0-10 ～1-20μg/mL . Thismethod has been usedto determinetrace oxalatein spinach and urine with satisfactory results.