中华鲟组织培养的初步研究

PRELIMINARY RESEARCH ON TISSUE CULTURE OF CHINESE STURGEON

取中华鲟的尾鳍、吻端、性腺等八种组织进行体外培养,得到来源于吻端和性腺组织的两个细胞株,分别命名为CSSn和CSG.CSSn和CSG均以成纤维样细胞为主。两种细胞都含有微染色体,染色体数目众多,在分布上具有“双峰多态”的特点,表明细胞已属异倍体。细胞生长较慢,CSG接种9d后、CSSn接种14d后数目达到最高值。CSG在16—30℃生长正常,最佳生长温度在27℃,最适pH为7.2。

Cell strains were established from snout and gonad tissues of Chinese sturgeon, Acipenser Sinensis, among eight tissues for primary culture and designated CSSn and CSG, respectively. Biological characteristics were partly examined for CSSn and CSG. Both strains appeared fibroblastic. They had large quantities of chromosomes and possessed microchromo-somes. The distributions of their chromosome number exhibited the same feature described as 'two wave crests distinguished and a great variety of distribution' , which demonstrated that they became aneuploid. Growth curve of both strains showed their relatively slow replication. The highest cell number of CSG was observed after incubated 9 days, while CSSn, 14 days. The effects of temperature and pH on growth were examined for CSG. CSG remained a normal state at 16 - 30℃, with optimum temperature of 27℃, and pH condition of 7.2.