摘要
Objective To explore the main pathogenic factors in the development of neuronal death during normothermic reperfusion in rabbits.Methods Ninety six New Zealand rabbits were randomly allocated into two groups: group Ⅰ served as non ischemic controls; group Ⅱ served as postischemic normothermic reperfusion models. Complete cerebral ischemia was induced by the four vessel model for 30 minutes. After ischemia, rabbits in group Ⅱ were further divided into three subgroups according to the duration of reperfusion: subgroup A, 30 minutes; subgroup B, 180 minutes and subgroup C, 360 minutes. Twenty eight biochemical parameters in the brain were measured, and neuronal changes were observed by histomorphological assessment. Neurons of 12 regions were differentiated into four types: type A (normal), type B (mildly damaged), type C (severely damaged) and type D (necrotic). Bivariate correlate analysis between the levels of biochemical parameters and the percentages of each type of neurons was carried out.Results The main parameters involved in the progressive decrement of type A neurons were VIP, β EP, PGI 2, T 3, T 4 and N + a,K + ATPase; in the increment of type B were β EP and TXB 2; in the increment of type C were GLU and TXB 2/PGI 2 respectively; in the stepwise increment of percentages of type D neurons were T 4, N + a,K + ATPase, GLU, T 3 and VIP (P<0.05).Conclusion The main factors involved in the development of neuronal death during postischemic normothermic reperfusion in rabbits include hypermetabolism, deactivation of N + a,K + ATPase, release of excitatory amino acids and disorder of neuropeptides.
Objective To explore the main pathogenic factors in the development of neuronal death during normothermic reperfusion in rabbits.Methods Ninety six New Zealand rabbits were randomly allocated into two groups: group Ⅰ served as non ischemic controls; group Ⅱ served as postischemic normothermic reperfusion models. Complete cerebral ischemia was induced by the four vessel model for 30 minutes. After ischemia, rabbits in group Ⅱ were further divided into three subgroups according to the duration of reperfusion: subgroup A, 30 minutes; subgroup B, 180 minutes and subgroup C, 360 minutes. Twenty eight biochemical parameters in the brain were measured, and neuronal changes were observed by histomorphological assessment. Neurons of 12 regions were differentiated into four types: type A (normal), type B (mildly damaged), type C (severely damaged) and type D (necrotic). Bivariate correlate analysis between the levels of biochemical parameters and the percentages of each type of neurons was carried out.Results The main parameters involved in the progressive decrement of type A neurons were VIP, β EP, PGI 2, T 3, T 4 and N + a,K + ATPase; in the increment of type B were β EP and TXB 2; in the increment of type C were GLU and TXB 2/PGI 2 respectively; in the stepwise increment of percentages of type D neurons were T 4, N + a,K + ATPase, GLU, T 3 and VIP (P<0.05).Conclusion The main factors involved in the development of neuronal death during postischemic normothermic reperfusion in rabbits include hypermetabolism, deactivation of N + a,K + ATPase, release of excitatory amino acids and disorder of neuropeptides.
