摘要
Objective To investigate the effect of cytokines on in vitro bone resor ption by cells isolated from giant cell tumor of bone.Methods Mononuclear stromal cells and multinucleated giant cells (MGC) were isolated from 11 cases of giant cell tumor of bone (GCT) and their bone res orption capability in an in vitro cell bone resorption model were tested. Expre ssions of some cytokines were detected by immunohistochemisty, Western blotting analysis and Enzyme linked immunoabsorbent assay (ELISA) in the GCT.Results The results showed that MGCs of GCT had capability to resorb bo ne matrix directly. Fibroblast like stromal cells (FC) could not only resorb bo ne matrix directly, but also secret unknown factors to facilitate bone resorptio n of MGC. Exogenous TNF α could significantly increase the bone resorption by both kinds of stromal cells, while exogenous IL 1 did not. Expression rate of M CSF and level of TNF α in GCT were higher than in osteosarcoma and normal se rum.Conclusions The characteristic bone resorption behavior of GCT might be caused by its three major cell components. The M CSF and TNF α could promote their bone resorption capability.
Objective To investigate the effect of cytokines on in vitro bone resor ption by cells isolated from giant cell tumor of bone.Methods Mononuclear stromal cells and multinucleated giant cells (MGC) were isolated from 11 cases of giant cell tumor of bone (GCT) and their bone res orption capability in an in vitro cell bone resorption model were tested. Expre ssions of some cytokines were detected by immunohistochemisty, Western blotting analysis and Enzyme linked immunoabsorbent assay (ELISA) in the GCT.Results The results showed that MGCs of GCT had capability to resorb bo ne matrix directly. Fibroblast like stromal cells (FC) could not only resorb bo ne matrix directly, but also secret unknown factors to facilitate bone resorptio n of MGC. Exogenous TNF α could significantly increase the bone resorption by both kinds of stromal cells, while exogenous IL 1 did not. Expression rate of M CSF and level of TNF α in GCT were higher than in osteosarcoma and normal se rum.Conclusions The characteristic bone resorption behavior of GCT might be caused by its three major cell components. The M CSF and TNF α could promote their bone resorption capability.
