摘要
Objectives To investigate the effects of erythromycin on the development of pulmonary fibrosis (PF) and to explore a new therapy in PF, the rat model of PF induced by bleomycin was used to investigate the effects of erythromycin on t he development of PF and its probable mechanisms Methods By evaluating the pathologic changes, the synthesis of collagen p rotein, the activity of tumor necrosis factor (TNF) and the expression of TNF mRNA, the effects of erythromycin on PF were investigated on the rat model of PF induced by bleomycin The alveolitis and different stages of PF we r e proven by pathology The contents of collagen protein of lung tissues were ca l culated by measuring the contents of hydroxyproline The activities of TNF s ecreted by macrophages and the expressions of TNF mRNA in lung tissues were determined Results 28 rats given bleomycin had all alveolitis of different degree af ter one week and pulmonary fibrosis after four weeks The degree of alveolitis after one week and fibrosis after four wee ks of the erythromycin group were alleviated in comparison with the control grou p by observation the pathology after HE stain, Masson stain and specific stain f or combined collagenous fibers After four weeks of Erythromycin treatment, the contents of collagen protein decreased (9884481 vs 142451385, t test, P<001) in comparison with the control group The macrophages of th e normal group almost did not secrete TNF After one week, the activiti es o f TNF secreted by the macrophages in both the control group and the erythrom ycin group increased, and the erythromycin group was higher than the control gro up There was no expression of TNF mRNA in lung tissues in the normal grou p But the rat lung tissues of the control group and the erythromycin group i n one week did express TNF mRNA, and the expressions of the erythromycin gro up were much more than the control group Conclusion Erythromycin may alleviate the process of PF induced by bleomy cin and its mechanism might not be related to the role of TNF
Abstract Objectives To investigate the effects of erythromycin on the development of pulmonary fibrosis (PF) and to explore a new therapy in PF, the rat model of PF induced by bleomycin was used to investigate the effects of erythromycin on t he development of PF and its probable mechanisms Methods By evaluating the pathologic changes, the synthesis of collagen p rotein, the activity of tumor necrosis factor (TNF) and the expression of TNF mRNA, the effects of erythromycin on PF were investigated on the rat model of PF induced by bleomycin The alveolitis and different stages of PF we r e proven by pathology The contents of collagen protein of lung tissues were ca l culated by measuring the contents of hydroxyproline The activities of TNF s ecreted by macrophages and the expressions of TNF mRNA in lung tissues were determined Results 28 rats given bleomycin had all alveolitis of different degree af ter one week and pulmonary fibrosis after four weeks The degree of alveolitis after one week and fibrosis after four wee ks of the erythromycin group were alleviated in comparison with the control grou p by observation the pathology after HE stain, Masson stain and specific stain f or combined collagenous fibers After four weeks of Erythromycin treatment, the contents of collagen protein decreased (9884481 vs 142451385, t test, P<001) in comparison with the control group The macrophages of th e normal group almost did not secrete TNF After one week, the activiti es o f TNF secreted by the macrophages in both the control group and the erythrom ycin group increased, and the erythromycin group was higher than the control gro up There was no expression of TNF mRNA in lung tissues in the normal grou p But the rat lung tissues of the control group and the erythromycin group i n one week did express TNF mRNA, and the expressions of the erythromycin gro up were much more than the control group Conclusion Erythromycin may alleviate the process of PF induced by bleomy cin and its mechanism might not be related to the role of TNF
