慢传输型便秘结肠肌间丛NOS、SOM、5-HT免疫反应性变化

Change of NOS, SOM and 5 HT immunoreactivity in myenteric plexus of sigmoid colon of patients with slow transit constipation

目的 探讨一氧化氮（ＮＯ） 、生长抑素（ＳＯＭ）及５羟色胺（５ＨＴ） 在慢传输型便秘（ＳＴＣ） 发病中的作用。方法 用免疫组织化学法结合计算机辅助的图像分析技术检测１５ 例ＳＴＣ 患者乙状结肠肌间丛一氧化氮合酶（ＮＯＳ） 、ＳＯＭ 及５ＨＴ的免疫反应性变化。结果 与对照组相比，ＳＴＣ 患者乙状结肠肌间丛ＮＯＳ、５ＨＴ免疫反应性增强（ Ｐ＜ ０－０１ ，Ｐ＜ ０－０５） ，而ＳＯＭ 无显著差别（ Ｐ＞ ０－０５）。结论 ＮＯＳ及５ＨＴ 免疫反应性增强是ＳＴＣ 患者结肠传输减慢的神经病理基础，ＳＯＭ 在ＳＴＣ结肠动力减弱中的作用不明显。

Objective To explore the role of nitric oxide(NO), somatostatin (SOM) and 5 hydroxytryptamine (5 HT) in pathogenesis of slow transit constipation (STC). Methods By using immunohistochemistry and computer assistant image analysis system, NOS, SOM and 5 HT immunoreactivity were examined in myenteric plexus of sigmoid colon resected from 15 patients with STC. Results Comparison with the controls, the immunoreactivity of NOS and 5 HT was increased significantly at myenteric plexus of STC, and no significant difference was determined in SOM. Conclusion The increase of immunoreactivity of NOS and 5 HT is the physiopathology basis of STC, and SOM has no obvious effect on the slow colonic transit.