一氧化氮与白介素2、8对肝硬化门脉系统血流量的影响

Effects of Nitric Oxide, Interleukin 2 and Interleukin 8 on Portal Hemodynamics of Cirrhotic Patients

目的：探讨一氧化氮（ＮＯ）、白介素２（ＩＬ－２及白介素８（ＩＬ－８）对肝硬化门静脉系统血流量的影响。方法：应用ＭＴＴ法检测肝硬化患者血清ＩＬ－２活性；应用ＥＬＩＳＡ法测定血清ＩＬ－８含量；应用荧光比色法检测血清ＮＯ水平；应用多普勒超声检测门静脉系统血流量参数。结果：肝硬化患者血ＩＬ－２活性、血清ＩＬ－８与ＮＯ水平显著高于正常对照组，其脾静脉血流量（ＳＶＦ）与肠系膜上静脉血流量（ＳＭＶＦ）之和亦显著大于正常对照组的门静脉血流量（ＰＶＦ）。结论：肝硬化患者血ＩＬ－２、ＩＬ－８增加可能为ＮＯ增多的诱发因素；肝硬化患者存在内脏高动力循环状态，ＮＯ增加可能起重要作用。

Background/Aims: To evaluate the effects of nitric oxide (NO), interleukin 2 (IL-2), and interleukin 8 (IL-8) on portal venous system flow in patients with cirrhosis. Methods: Serum IL-2 activity and IL-8 levels were determined in cirrhotic patients by using MTT method and ELISA assay; Serum NO concentration was also measured by using a fluorometric assay, The parameters of portal venous system flow were determined by Doppler ultrasonography. Results: Concentration of serum IL- 8, NO and IL-2 activity in cirrhotic patients were markedly higher than those in healthy volunteers. The sum of splenic venous flow (SVF) and superior mesenteric venous flow (SMVF) in patients with hepatic cirrhosis was greater than portal venous flow (PVF) in normal controls. Conclusions: Splanchnic hyperdynamic circulatory state associated with portal hypertension was observed in all patients with cirrhosis. The excessive production of NO induced by interleukins is responsible for the hyperdynamic circulation in patients with cirrhosis.