高压氧对兔眼视网膜血流量和细胞因子影响的实验研究

Experimental study of the effects of hyperbaric oxygen on retinal blood flow and cytokines in rabbit's eyes

目的 检测高压氧治疗前后兔眼玻璃体氧分压、内皮素、胰岛素样生长因子－ １ 及视网膜血流量的变化，探讨高压氧治疗视网膜疾病的机理，为高压氧治疗眼病提供理论根据。方法 用氩激光光凝法制作兔眼视网膜静脉阻塞眼模型，血气分析法测定玻璃体氧分压，放射微球标记法检测视网膜血流量；放射免疫分析法测定玻璃体内内皮素含量，酸酒精提取法和免疫放射测定分析法检测玻璃体内胰岛素样生长因子－ １ 的含量。经统计学处理分析其相互关系，提出高压氧治疗视网膜疾病的作用机理。结果 高压氧治疗前后视网膜中央静脉阻塞眼玻璃体氧分压由１２６０±１９２ｋＰａ 升为２３３４±２００ｋＰａ（Ｐ＜００１） ；视网膜血流量由３３８±１５１ｍＬ·ｍｉｎ－１ ·ｇ －１降低为０２５±００８ｍＬ·ｍｉｎ－１ ·ｇ－ １（Ｐ＜ ００１）； 玻璃体内皮素含量经３～５ 次高压氧治疗后， 由０升高为８２９９±２５２３ｎｇ·Ｌ－１（Ｐ＜００１）；玻璃体内胰岛素样长生因子－１ 含量由７４２±１１８ｎｇ·Ｌ－１ 升高为１９８１±１４１ｎｇ ·Ｌ－１（Ｐ＜００１）。结论 高压氧有提高玻璃体氧分压， 增加氧储备作用；高氧可明显降低视网膜血流量，提示：在高压氧治疗时必?

Objective Detecting vitreous oxygen pressure(PO2),endothelins(ETs),insulin-like growth factor-1(IGF-1)and retinal blood flow in rabbit's eye before and after hyperbaric oxygen treatment(HBOT),in order to study the mechanism of HBOT in retinal disease.Methods Experimental retinal vein occlusion(RVO) eyes were done by Argon laser.vitreous PO2 value was determined by blood gas analysis;retinal blood flow was measured with radiolabelled microspheres method;vitreous ETs were detected by Radioimmunoassay;vitreous IGF-1 value was acquired with acid-ethanol extraction and immunoradiometric assay.Results Vitreous PO2 value increased from 12.60±1 92kPa before HBOT to 23.34±2.00kPa after HBOT(P<0 01);Retinal blood flow decreased from 3.38±1.51mL ·min-1 ·g-1to 0.25±0.08mL·min-1·g-1(P<0 01);Vitreous ETs increased from 0 to 82.99±25.23ng ·L-1 after 3～5HBOT(P<0 01);Vitreous IGF-1 increased from 7.42±1.18ng·L-1 to 19.81±1.41ng ·L-1 (P<0 01) Conclusion HBO can increase vitreous PO2 and storage of oxygen;HBO decrease retinal blood flow obviously,so we must improve blood flow in HBOT; Vitreous ETs and IGF-1 values are obviously increased after 3～5 HBOT,these factors are activated by HBO and participated in ocularbiologic and pathologic process in HBOT according to their quantities,this is one of factors effecting on HBOT; HBO has direct and indirect treatments.Indirect treatment has two side,it's side effect is one of factors causing complications in HBOT,We must pay attention to it.