内皮素-1对肺泡Ⅱ型上皮细胞肺表面活性物质合成的调控

Modulation of endothelin 1 on pulmonary surfactant synthesis of cultured alveolar type Ⅱ cells

采用离体培养的肺泡Ⅱ型上皮细胞（ＡＴⅡ）观察内皮素１（ＥＴ１）对肺表面活性物质（ＰＳ）合成的影响。结果显示：１０－１１～１０－８ｍｏｌ·Ｌ－１的ＥＴ１可促进ＰＳ的合成，量效关系显著。与肺组织块培养相比，促进ＡＴⅡ细胞ＰＳ合成所需的ＥＴ１浓度较大。ＥＴ１的促ＰＳ合成效应可被ＥＴＡ受体拮抗剂ＢＱ１２３阻断，而不受ＥＴＢ受体拮抗剂ＢＱ７８８影响。ＥＴ１作用１６ｈ对ＡＴⅡ型细胞的增殖无显著影响。结果表明，ＥＴ１既可通过ＥＴＡ受体直接促进ＡＴⅡ细胞合成ＰＳ。

The effects of endothelin 1(ET 1) on pulmonary surfactant(PS) synthesis of cultured ATⅡ cells were observed. The results showed that: ① ET 1(10 -11 ～10 -8 mol·L -1 ) enhanced PS synthesis of cultured ATⅡ cells in a dose dependent manner. ② The minimum effective concentration of ET 1 enhanced PS synthesis of cultured ATⅡ cells was higher than that of lung explants. ③ ET A antagonist BQ 123 decreased the effect of ET 1 on PS synthesis, but ET B antagonist BQ 788 did not change the effect. ④ ET 1 (10 -12 and 10 -10 mol·L -1 ) had no effect on the proliferation of ATⅡ cells. These results confirmed that ET 1 can enhance directly and indirectly on the PS synthesis of ATⅡ cells mediated via ET A receptor. The effect of ET 1 on PS synthesis was not induced by changing the number of ATⅡ cells.