维生素C对沙眼衣原体增殖的影响及其应用

Effects and Applications of Ascorbic Acid on the Proliferation of Chlamydia trachomatis

采用细胞培养法和免疫荧光法，对２８８份临床标本进行沙眼衣原体（ＣＴ）的分离鉴定，并观察维生素Ｃ（ｖｉｔＣ）对阳性标本中ＣＴ包涵体形成的影响。结果显示ＣＴ在ｖｉｔＣ５μｇ·ｍｌ－１组中包涵体形成率为２９５％，与含放线菌酮的对照组比较差异无显著性（Ｐ＞０．０５）。提示ｖｉｔＣ是ＣＴ所需要的一种营养物质，可促进ＣＴ在细胞内的增殖；

Two hundred and eighty eight samples of patients with nongonococcal urethritis (NGU) and prostatitis were detected by cell culture and immunofluorescence assay for Chlamydia trachomatis (CT) and the effects of ascorbic acid(vitC) on the formation rate of inclusion of CT in positive samples were also studied. The results showed that the formation rate of inclusion of CT was 29.5% when the concentration of vitC was 5μg·ml -1 . The difference between test group and control group which contained cycloheximide in the media was insignificant ( P >0.05). The results suggested that vitC was a kind of nutrient needed for CT. The proliferation of CT in the cell can be promoted by vitC. We can replace cycloheximide by vitC in McCoy cell culture to detect clinic samples with CT.