L—谷氨酰胺发酵液预处理技术

Studies on the Pretreatment Technology of L-glutamine Fermentation Broth

研究了谷氨酰胺发酵液在浓缩结晶前及结晶母液的预处理技术,提出了在50℃下加入最终浓度为100 mg/L絮凝剂和100 mg/L助凝剂,待发酵液混凝后再进行3 000 r/min低速离心的预处理方法,可有效除去发酵液中的菌体等固形物。对原提取工艺中废弃的一次母液,作者提出对其超滤后再进行二次浓缩结晶的处理方法,可以从中再提取到约60%的谷氨酰胺。此外,还对一些异常发酵产生的发酵液的处理方法进行了初步研究。

The pretreatment technology of L-glutamine fermentation broth before concentration crystallization and in the first mother liquor were studied. Under 50℃, 100 mg/L flocculating agent and 100 mg/L assistant flocculating agent and the centrifugation by 3,000 r/min,the solid components such as the bodies of microorganisum could be removed effectively. Not as same as the abandon in the original technology,the first mother liquor as concentrated to affect the second crystallization after being ultrafiltered. Then 60% glutamine in the first mother liquor could be obtained. In addition, some fermentation liquor produced by conceptional fermentation was studied.