摘要
A novel asy interacting protein gene (asyip) was isolated from human lung cell line(WI-38) cDNA library with yeast two-hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of 1.8 kb and 2.7 kb in various human tissues at differential level. Putative ASYIP protein contains two large high hydrophobic regions, two protein kinase C phosphorylation sites and two casein kinase I phosphorylation sites. A endoplasmic reticulum retrieval signal (Lys-Lys-Lys-Ala-Glu) is present at the C-terminal of ASYIP protein. Interaction of ASYIP and ASY in mammalian cell was further identified by co-immunoprecipitation assay. Like asy gene, overexpression of asyip gene can inhibit growth of tumor cell Saos-2 and induce cell apoptosis, but the efficiency is lower than that of asy gene.
A novel asy interacting protein gene (asyip) was isolated from human lung cell line(WI-38) cDNA library with yeast two-hybrid system. The asyip gene is constitutively expressed as two mRNA transcripts with the size of 1.8 kb and 2.7 kb in various human tissues at differential level. Putative ASYIP protein contains two large high hydrophobic regions, two protein kinase C phosphorylation sites and two casein kinase I phosphorylation sites. A endoplasmic reticulum retrieval signal (Lys-Lys-Lys-Ala-Glu) is present at the C-terminal of ASYIP protein. Interaction of ASYIP and ASY in mammalian cell was further identified by co-immunoprecipitation assay. Like asy gene, overexpression of asyip gene can inhibit growth of tumor cell Saos-2 and induce cell apoptosis, but the efficiency is lower than that of asy gene.
