RG50864对PDGF诱导的PASMC DNA含量及增殖细胞核抗原表达的影响

The Effects of RG50864 on the Expression of the Percent Content of PDGF Induced PASMC DNA and Proliferation Cells Nuclear Antigen

目的：研究酪氨酸蛋白激酶抑制剂ＲＧ５０８６４对ＰＤＧＦ诱导的肺动脉平滑肌细胞（ＰＡＳＭＣ）ＤＮＡ含量及增殖细胞核抗原表达的影响。方法：应用原代培养的小牛肺动脉平滑肌细胞，用流式细胞仪分析ＲＧ５０８６４对ＰＤＧＦ诱导的ＰＡＳＭＣＤＮＡ百分含量及增殖细胞核抗原的变化。结果：与对照组相比，ＲＧ５０８６４处理组可显著地抑制ＰＤＧＦ诱导的ＰＡＳＭＣ增殖细胞核抗原表达的荧光强度及荧光指数（Ｐ＜０．００１），增加ＰＡＳＭＣ生长周期中的Ｇ０／Ｇ１期的ＤＮＡ百分含量，抑制Ｓ期及Ｇ２Ｍ期ＤＮＡ百分含量（Ｐ＜０．０１或Ｐ＜０．００１）。结论：ＲＧ５０８６４可显著地抑制ＰＤＧＦ诱导的ＰＡＳＭＣ增殖细胞核抗原表达，抑制ＰＡＳＭＣ周期Ｓ期及Ｇ２Ｍ期ＤＮＡ百分含量，阻止ＰＡＳＭＣ周期由Ｇ０／Ｇ１向ＤＮＡ合成的Ｓ期及Ｇ２Ｍ期转化。

The study was designed to explore the effects of RG50864 on DNA percent and proliferation cell nuclear antigen (PCNA) of PDGF induced pulmonary artery smooth muscle cells (PASMC).Methods:By primary culture of calf PASMC,the changes of RG50864 on DNA percent content and PCNA of PDGF induced PASMC were observed by using flow cytometry.Results:As compared with control group,fluorescence intensity and index of PDGF induced PASMC PCNA expression were significantly inhibited by RG50864 groups ( P<0.001 ),DNA percent content of G 0/G 1 phase was increased,however,which of S and G 2M phase was decreased in RG50864 groups ( P <0.1 or P<0 001 ).Conclusion:RG50864 could significantly inhibite the PCNA expression of PDGF induced PASMC,and block the converting of cells from G 0/G 1 phase to S phase of DNA synthesis.