Calponin对体外培养的自发性高血压大鼠血管平滑肌细胞增殖的抑制作用
摘要
为观察Calponin对血管平滑肌细胞增殖的抑制作用,将用liposome+pCAGGS质粒介导的人重组Calponin基因(hCN)导入体外培养的第3代自发性高血压大鼠(SHR)血管平滑肌细胞(VSMC)内,发现hCN可在VSMC内持续表达4周以上。MTT检测结果显示,导入的hCN基因能明显抑制SHR的VSMC增殖。这种作用对血管成形术后再狭窄的防治和高血压时血管壁肥厚的逆转具有潜在的实用价值。
参考文献6
-
1Ross R.A problem of the biology of arterial wall cell and their interaction with blood components. Atherosclerosis . 1981
-
2Chomczynski P.A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques . 1993
-
3Plautz GE,Nabel EG,Nabel GJ.Introduction of vascular smooth muscle cells expressing recombinant genes in vivo. Circulation . 1990
-
4Takahashi K,Fukui R,Kato O, et al.Percutaneous transluminal transfer of the human calponin gene for suppression of intimal hyperplasia following arterial balloon injury: a model for successful gene therapy for restenosis. Circulation . 1993
-
5Takahashi K,Hiwada K,Kokubu T.Isolation and characterization of a 34 000 -dalton calmodulin-and F-actin-binding protein from chicken gizzard smooth muscle. Biochemical and Biophysical Research Communications . 1986
-
6Negoro N,Fukui R,Tsuchikane E et al.Down expression of calponin in smooth muscle of coronary artery lesion identifies a group of lesion at high risk for restenosis after athetectomy. Circulation . 1994
-
1苗志林,韩雅玲,王守力,荆全民,李菲,王冬梅,刘民培.Calponin对培养的自发性高血压大鼠血管平滑肌细胞增殖的作用[J].解放军医学杂志,1998,23(6):425-427. 被引量：2
-
2高志良,姚集鲁,吕凌,陈文思.不同外周血标本中HCV RNA检出比较[J].中华传染病杂志,1994,12(1):29-32. 被引量：8
-
3苗志林,韩雅玲,王守力,刘莹,刘民培,王承利.用infiltrator导管向兔髂动脉壁内导入calponin基因的效果[J].第四军医大学学报,2000,21(10):1268-1270.
-
4王莹,王广.“生命网”在冠心病患者二级预防中的有益作用[J].山东医药,2009,49(20):8-9. 被引量：3
-
5Jian QIU,Xiao-hui WEI,Fang GENG,Rui LIU,Jing-wu ZHANG,Yu-hong XU.Multivesicular liposome formulations for the sustained delivery of interferon α-2b[J].Acta Pharmacologica Sinica,2005,26(11):1395-1401. 被引量：11
-
6李刚,孟立平,周昌钻,蒋承建,郭艳,池菊芳,郭航远.同型半胱氨酸对大鼠主动脉血管平滑肌细胞表型转化的影响[J].温州医科大学学报,2016,46(1):33-38. 被引量：3
-
7陈啟炜,杨勇,陈兵,陈杨,李胜,王桂兰,吴玉龙,陈崇会,祖宝利,易斌,鲁开智,廖林.梗阻性黄疸患者血清对人肺微血管内皮细胞肌样分化的影响[J].中华麻醉学杂志,2016,36(9):1146-1149.
-
8王书美,吕丹,陈炜,张晓娟,曹兴水,张连峰.心脏特异表达Calponin 1转基因小鼠的心脏功能分析[J].中国比较医学杂志,2011,21(1):59-63. 被引量：1
-
9YuanQiong-ying,LiuHou-yu,ZhouKang,ShiBi-jian.Preparation and Pharmacokinetics of Giycyrrhizin Liposome[J].胃肠病学,2000,5(B08):186-186.
-
10杨博,李平,孟立平,郭航远.瑞舒伐他汀抑制同型半胱氨酸诱导的大鼠主动脉血管平滑肌细胞表型转化及信号通路[J].西安交通大学学报（医学版）,2016,37(4):506-512. 被引量：4
