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固相萃取高效液相色谱法测定血浆霉酚酸含量

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摘要 为监测肾移植术后患者血中毒酚酸(MPA)浓度,用固相萃取法处理作品,以泼尼松为内标物,甲醇:四氢呋喃:水作流动相,采用反相高效液相色谱法测定血浆游离MPA浓度。经方法学评价血浆MPA回收率为92.3%~103.1%,绝对回收率50.2%~53.7%。MPA浓度在0.2~40μg/ml范围内呈良好线性关系,最低检测极限为0.2μg/ml。批内与日间相对标准差<3.3%和<4.7%。结果表明本法用于MPA测定具有准确、灵敏、简便等特点。
出处 《沈阳部队医药》 1999年第5期404-407,共4页
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