杂色曲霉产毒培养物的诱变性研究

Mutagenic Study on Aspergillus versicolor Toxigenic Cultures

杂色曲霉产毒培养物提取液的Ａｍｅｓ试验结果显示，４株杂色曲霉产毒培养物提取液，在不加和加Ｓ－９活化两种条件下，其中２株产毒培养物提取液对鼠伤寒沙门氏菌组氨酸营养缺陷型菌株ＴＡ９８和ＴＡ１００显示致诱变性，各剂量组回变菌落数为阴性对照的２～１４．２倍，且剂量与效应呈正相关（ＴＡ９８ｒ＝０．８８３～０．９９８，ＴＡ１００ｒ＝０．９７３～１．０）。在加入Ｓ－９活化条件下，各剂量组回变菌落数是－Ｓ９时的１．４～２．９倍。说明杂色曲霉产毒培养物经微粒体酶代谢系统活化后，毒性作用增强。

The mutagenicity of the toxigentic culture extracts of 4 stais of A. versicolor were detected by Ames test.The results showed that two extracts could result reverse mutation of the Salmonella typhimurium TA98 and TA100 with histidine nutrition deficiency with S-9 influx or without.The colony formig units from reverse mutation for all dose groups in each plate exceeded the gegative control group's by 2～14.2 times,and the dose effect positive (TA98 r =0.883-0.998,TA100 r =0.973-1.0).The reverse mutation colony forming units for each dose group S-9 influx were 1.4～2.9 times as many as those without S-9.With results showed the toxicity of the toxigenic culture extracts od A.versicolor strains was increased by adding mammals microsomal enzyme system. e