摘要
Objective:To study thei nhibitory effect of purified trichosanthin component on the proliferation of malignant melanoma.Methods:The effect of purified trichosanthin componenton the DNA synthesis,cell cycle and cell apoptos is of murinemelanomacellsweredetected by flow cytometry when culture dinvitro.Results:The significantG0/G1phasearrest was revealed by the inc rease of cellsinG0/G1 phase and decrease of cell sinS phase.The obviousapoptosis of melanoma cells was in duced bypuri fied trichos anthin component.G0/G1phase arrest was high lycorrelated withapop to sis(r=0.8705).Conclusion:The purified trichos anthin component can markedly inhibit melanoma cells by the suppression of DNA syn the sisin S phaseand cell mitosisas well as induction of cell apoptosis.
Objective:To study thei nhibitory effect of purified trichosanthin component on the proliferation of malignant melanoma.Methods:The effect of purified trichosanthin componenton the DNA synthesis,cell cycle and cell apoptos is of murinemelanomacellsweredetected by flow cytometry when culture dinvitro.Results:The significantG0/G1phasearrest was revealed by the inc rease of cellsinG0/G1 phase and decrease of cell sinS phase.The obviousapoptosis of melanoma cells was in duced bypuri fied trichos anthin component.G0/G1phase arrest was high lycorrelated withapop to sis(r=0.8705).Conclusion:The purified trichos anthin component can markedly inhibit melanoma cells by the suppression of DNA syn the sisin S phaseand cell mitosisas well as induction of cell apoptosis.
