期刊文献+

Effect of Reduqing (热毒清) on Endotoxin-Induced Production of Tissue Factor and Cytokines in Human Whole Blood

Effect of Reduqing(热毒清) on Endotoxin-Induced Production of Tissue Factor and Cytokines in Human Whole Blood
原文传递
导出
摘要 Objective: TO study the effect of Reduqing (RDQ) Injection on the lipopolysaccharide (LPS)induced tissue factor and cytokine production in whole blood. Methods: Heparinized human blood was incubated with LPS in the presence or absence of RDQ. The plasma concentrations of TNF-α, IL-1β, IL-6, and IL-8were measured by enzyme-linked immunosorbent assays (ELISA) and the monocyte tissue factor activity wasmeasured by a one-stage tissue factor induced plasma clotting time assay. Results: RDQ was found to diminishthe LPS-induced increase of TNF-α, IL-1β and IL-6 in plasma but did not completely abolish their production.In contrast to the effect on these cytokines, RDQ caused further increase of the plasma level of IL-8 and themonocyte TF activity in the presence of LPS. Conclusions: In the in vztro whole blood assay system used inthis study, the decrease of LPS-induced production of TNF-α, IL-1β, and IL-6 was similar to a previous in vivostudy on the effect of RDQ on the production of these cytokines in response to two-time LPS injection in rabbits, while the increase of IL-8 and TF production was contradictory to the previous in vivo study. Potentialreasons contributing to the differences are discussed. Objective: TO study the effect of Reduqing (RDQ) Injection on the lipopolysaccharide (LPS)induced tissue factor and cytokine production in whole blood. Methods: Heparinized human blood was incubated with LPS in the presence or absence of RDQ. The plasma concentrations of TNF-α, IL-1β, IL-6, and IL-8were measured by enzyme-linked immunosorbent assays (ELISA) and the monocyte tissue factor activity wasmeasured by a one-stage tissue factor induced plasma clotting time assay. Results: RDQ was found to diminishthe LPS-induced increase of TNF-α, IL-1β and IL-6 in plasma but did not completely abolish their production.In contrast to the effect on these cytokines, RDQ caused further increase of the plasma level of IL-8 and themonocyte TF activity in the presence of LPS. Conclusions: In the in vztro whole blood assay system used inthis study, the decrease of LPS-induced production of TNF-α, IL-1β, and IL-6 was similar to a previous in vivostudy on the effect of RDQ on the production of these cytokines in response to two-time LPS injection in rabbits, while the increase of IL-8 and TF production was contradictory to the previous in vivo study. Potentialreasons contributing to the differences are discussed.
出处 《Chinese Journal of Integrative Medicine》 SCIE CAS 1998年第1期43-47,共5页 中国结合医学杂志(英文版)
关键词 REDUQING tissue factor tumor necrosis factor-α INTERLEUKIN-1Β interleukin-6 interleukin-8 lipopolysaccharide MONOCYTES mononuclear cells Reduqing, tissue factor, tumor necrosis factor-α, interleukin-1β, interleukin-6, interleukin-8,lipopolysaccharide, monocytes, mononuclear cells
  • 相关文献

参考文献7

二级参考文献2

共引文献35

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部