不同年龄帕金森病小鼠模型脊髓前角细胞的超微结构变化

Changes of ultrastructure of spinal cord anterior horn cells in Parkinson′s disease mice model of different age

目的观察MPTP诱导的帕金森病(PD)模型小鼠脊髓前角的结构变化及其与年龄的关系。方法 C57BL/6J小鼠分为8周龄正常对照组,8周龄模型组,18月龄正常老龄对照组,18月龄老龄模型组。连续5 d给药并进行行为学观察后取小鼠颈膨大和腰膨大电镜下观察超微结构。结果 MPTP诱导PD小鼠脊髓超微结构呈病理改变:神经元水样变性明显,可见较多胀亡和凋亡神经元;星形胶质细胞增生肥大及小胶质细胞活化;广泛出现血脑屏障(BBB)通透性增加。以上病理改变在老龄组的更加显著。结论年龄老化是帕金森病不可忽略的影响因素。脊髓前角的病理性变化是可能PD运动障碍的原因和结构基础之一,PD的病变部位可能更加广泛。

Objective To observe alterations of cornu anterius medullae spinalis structure and glial fibrillary acid protein（GFAP） and explore the relationship between the alterations with age in 1-methyl-l-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）-induced Parkinson′s disease（PD） model mice.Methods C57BL/6J mice（n=32） were divided into the following groups： PD model group of 8 weeks old,control group of 8 weeks old,PD model group of 18 months old and control group of 18 months old.PD model mice were injected intraperitoneally with 30 mg/kg MPTP each day for 5 days.Control mice were only injected with normal saline.The cervical enlargement and intumescentia lumbalis of mice were taken out.The ultrastructural changes of cornu anterius medullae spinalis were observed under electronic microscope.Results Hydropic degeneration of neurons and apoptosis and oncosis could be found in general regions.Astrocytes were hyperplastia and hypertrophy.Meanwhile,gitter cells were activated.A serious of ultrastructural changes of permeability increasing of blood-brain barrier of MPTP-injected mice existed.Above pathological changes in old-age groups were more prominent.Conclusions Ageing is influencing factors for PD.Pathological alteration of cornu anterius medullae spinalis may be one of the reasons and the structure basis for the dyskinesia in PD.The regions of pathological changes of PD may be more widespread.