CD44^＋-ESA^＋在结肠癌干细胞分选中的应用

Role of CD44^＋/ESA^＋ in sorting colonic cancer stem cells

目的分选结肠癌细胞株SW480细胞中的CD133^＋-CD44^＋-ESA^＋亚群细胞，并观察其致瘤性。方法用流式细胞仪分选SW480细胞中CD133^＋．CD44^＋-ESA^＋,CD133^--CD44^＋-ESA^＋及CD133^--CD44^--ESA亚群细胞。将这3组细胞分别接种于NOD／SCID小鼠，每组5只。观察肿瘤生长情况。结果SW480细胞中CD133^-．CD44^--ESA^-、CD133^--CD44^＋-ESA^＋和CD133^＋．CD44^＋-ESA^＋亚群细胞分别占（86．38~10．23）％、（1．26~0．28）％和（0．38±0．07）％。接种3d后，CD133^＋．CD44^＋．ESA^＋细胞组小鼠可形成瘤结节：CD133^--CD44^＋-ESA^＋细胞组小鼠9d左右可见瘤结节．而CD133^--CD44^--ESA^-细胞组小鼠则要到15d方可见瘤结节。接种后18d，3组小鼠肿瘤大小分别为（13．82±5．04）mm^3、（9．25±4．57）mm^3和（4．76±3．92）mm^3，差异有统计学意义（P〈0．05）。结论CD44^＋-ESA^＋结肠癌干细胞表面标记之-：CD133^＋．CD44^＋-ESA^＋细胞则是SW480细胞的癌干样细胞．

Objective To isolate CD133^＋/CD44^＋/ESA^＋ subsets cells from SW480 colon cancer cells, and to observe the tumor formation. Method CD133^＋/CD44^＋/ESA^＋ subsets cells, CD133^-/ CD44^＋/ESA＋ subsets cells and CD133^-/CD44^-/ESA^- subsets cell were sorted by flow cytometry from SW480 colon cancer cells, then three subsets were separately inoculated in five NOD/SCID mice and the growth rates were calculated. Result The proportion of CD133^-/CD44^-/ESA^-, CD133^-/CD44^＋/ESA^＋ and CD133＋/CD44＋/ESA^＋ subsets cells in SW480 cells were （86.38±10.23）%, （1.26±0.28）% and （ 0.38±0.07 ） %. After inoculation, tumor nodules could be formed three days later in CD 133 ^＋/CD44^＋/ESA＋ group, and they could be formed 9 days later in CD133^-/CD44^＋/ESA^＋ group, while they could be formed 15 days later in CD133^-/CD44^-/ESA^- group. Eighteen days later, tumor sizes in three groups were （13.82±5.04） mm^3, （9.25±4.57） mm^3 and （4.76±3.92） mm^3 respectively, and the differences were statistically significant （P〈0.05）. Conclusion ESA^＋-CD44^＋ is one of the surface markers for colonic cancer stem cells, and CD133^＋-CD44^＋-ESA^＋ cells are SW480-like cancer stem cells.