人重组白介素6对人结肠癌HT-29细胞黏蛋白表达的调控

Expression of mucin regulated by recombinant human interleukin-6 in HT-29 cell of colon cancer

目的研究人重组白介素6（rIL-6）对人结肠癌HT-29细胞中黏蛋白表达的调控，以期对结肠癌的发生发展进一步了解。方法采用流式细胞技术和RT-PCR分别检测1、2、5、10和20μg/L的rIL-6作用于HT-29细胞后黏蛋白1和黏蛋白2表达变化，采用Transwell细胞侵袭实验研究rIL-6对HT．29细胞侵袭力的影响。结果2μg／L以上的rIL-6能够促进结肠癌HT-29细胞中黏蛋白1的表达，分别为（12．5±1．6）％、（26．6±2．7）％、（33．9±2．8）％和（58．9±2．5）％，均高于阴性对照组（8．0±0．8）％（P〈0．01）。反之，2μg／L以上的rIL-6可抑制黏蛋白2的表达，分别为（30．5±2．6）％、（17．0±2．7）％、（11．0±2．0）％和（5．3±1．8）％，均低于阴性对照组（41．6±3．6）％（P〈0．01）。随着rIL-6浓度的增加，HT-29细胞的透膜细胞数增加（P〈0．01）。结论rIL-6能促进结肠癌HT-29细胞中黏蛋白1的表达．抑制黏蛋白2的表达，对结肠癌细胞的浸润有促进作用。

Objective To study the regulation of the mucin protein in colon cancer cell line HT-29 by recombinant human interleukin-6（rIL-6） and to further elucidate the development of colon caner. Methods The HT-29 cells were treated with different concentrations of rIL-6 （1, 2, 5, 10 and 20 μg/L）, then flow cytometry and RT-PCR were used to detect the expression of mucinl and mucin2. Transwell invasion assay was used to observe the effect of invasion capability of rIL-6 to HT-29 cells. Results In colon cancer, the expression of mucin 1 could be promoted by rIL-6 with concentration above 2 μg/L, the expression rates were （12.5±1.6）%, （26.6±2.7）%, （33.9±2.8）% and （58.9±2.5）%, respectively, higher than （8.0-±0.8）% in the negative controls （P〈0.01）, meanwhile, the expression of mucin 2 decreased by rIL-6 with concentration above 2 p^g/L, the expression rates were （30.5±2.6）%,（17.0±2.7）%,（11.0±2.0）% and （5.3±1.8）%, respectively, lower than （41.6±3.6）% in negative control （P〈0.01）. With the increase in rIL-6 concentration, the invasion of HT-29 cells was enhanced. Conclusions In colon cancer, the expression of mucinl can be promoted by rIL-6, while the expression of mucin2 can be inhibited. IL-6 is a promoting effect factor in colon cancer invasion and metastasis.