常见的缺失型α地中海贫血2的基因检测

Detection of Common Deletional α Thalassemia 2 determinants

应用聚合酶链反应技术检测缺失型α地中海贫血－２。特异性引物选择性扩增α２和α１基因，ＰＣＲ产物经琼脂糖凝胶电泳后即可对－α３．７和－α４．２缺失型作出基因诊断。此方法简便，快速和可靠。本研究为在缺失型α地中海贫血－２高发区进行－α３．７和－α４．２缺失型的筛查和产前诊断提供了一个新的方法。

Using polymerase chain reaction to detect common deletional α thalassemia 2. Specific primers selectively amplify the α2 and α1 genes. After electrophoresis of PCR products, then can make diagnosis for genotypes of the α 3 7 and α 4 2 deletions. This method is simple, fast, reliable and non radioactive. It offers a new method for large screening and prenatal diagnosis of α 3 7 and α 4 2 deletions in the area with high frequency of deletional α thalassemia 2.