白细胞识别与计量生物传感器的研究

A Study on the Voltammetric Biosensor for Discriminating and Counting Human Leukocyte

介绍一种以平面热解石墨电极为工作电极，用于识别与计量健康人白细胞的半微分伏安型生物传感器。在本传感器上建立健康人淋巴细胞及粒细胞的识别模式，用本传感器计量细胞数，当活细胞数在（１．８～１８．１）×１０４ｃｅｌｓ范围内，循环扫描峰电流的半微分值与细胞数之间存在着良好的线性相关（ｒ＝０．９８８８）。

A Biosensor which can discriminate and count the human leukocyte is reported. The leukocyte on trap membrane applied to basal plane pyrolytic graphite electrode is detected with semi differential voltammetry in phosphate buffered solution. The model of discriminating human granulocyte and lymphocyte has been constructed. A good line correlation between cell numbers blocking trap membrane and semi differential peak current has been obtained in the range of cell numbers from 1 8×10 4 to 18 1×10 4 cells (r=0 988 8).